HPLC Linked Electrospray Tandem Mass Spectrometry: A Rapid and Reliable Method to Analyse Indole-3-Acetic Acid Metabolism in Bacteria

The developmental plasticity of plants according to changes in their growth conditions is mediated by signaling molecules called hormones. The major classes of plant hormones are auxin, gibberellins, abscisic acid, and ethylene. Among these, the most important is indole-3-acetic acid (IAA). Quantification of IAA in an extract of approximately 10 mg obtained from the cambial zone of Eucalyptus leaves was performed by ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS). The leaves were extracted using acetone, and the extract was analyzed using a reverse-phase column (Acquity UPLC BEH C18, 2.1 mm × 100 mm, 1.7 µm) at a flow rate of 0.2 mL/min with gradient elution of an aqueous mobile phase (containing 0.1% formic acid) with methanol. This gradient elution provided an excellent performance in terms of specificity/selectivity, linearity, precision, and ruggedness/stability. In addition, the run time was short (6 min), with excellent linearity (R2 > 0.99) in the range of 10–70 ng/mL. The structure of IAA was elucidated using UPLC/ESIMS/MS, operating and quantifying in multiple reaction monitoring (MRM) mode.

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Quantification of Indole-3-Acetic Acid and Amino Acid Conjugates in Rice by Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.69.778 ◽

2005 ◽

Vol 69 (4) ◽

pp. 778-783 ◽

Cited By ~ 54

Author(s):

Fumio MATSUDA ◽

Haruna MIYAZAWA ◽

Kyo WAKASA ◽

Hisashi MIYAGAWA

Keyword(s):

Mass Spectrometry ◽

Acetic Acid ◽

Liquid Chromatography ◽

Amino Acid ◽

Tandem Mass Spectrometry ◽

Electrospray Ionization ◽

Tandem Mass ◽

Amino Acid Conjugates ◽

Indole 3 Acetic Acid ◽

Ionization Tandem Mass Spectrometry

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Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

The Open Plant Science Journal ◽

10.2174/1874294701710010100 ◽

2017 ◽

Vol 10 (1) ◽

pp. 100-109

Author(s):

Hongxia Li ◽

Natasha Trzaskalski ◽

R.J. Neil Emery

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

High Performance ◽

Soybean Seeds ◽

Tandem Mass ◽

Electrospray Tandem Mass Spectrometry ◽

Aqueous Methanol ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

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